beta-D-吡喃葡萄糖基-(1->3)-beta-D-吡喃葡萄糖基-(1->3)-beta-D-吡喃葡萄糖基-(1->3)-D-吡喃葡萄糖合成路线 （共 23 条）
反应条件
potassium dihydrogenphosphate; Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa in aq. acetate buffer T=30°C; pH=5.5; Enzymatic reaction; Equilibrium constant;
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
1: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction 2: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction View Scheme
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide in water T=45°C; pH=7; 0.166667 h; Enzymatic reaction;
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
1: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction 2: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction View Scheme
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
1: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction 2: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction View Scheme
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
1: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction 2: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction View Scheme
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
1: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction 2: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; Cleland's reagent / aq. acetate buffer / 0.33 h / 30 °C / |Enzymatic reaction View Scheme
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
potassium dihydrogenphosphate; Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa in aq. acetate buffer T=30°C; pH=5.5; Enzymatic reaction; Equilibrium constant;
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
反应条件
1: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction 2: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction View Scheme
参考文献
Kitaoka, Motomitsu; Matsuoka, Yasuyuki; Mori, Kiyotaka; Nishimoto, Mamoru; Hayashi, Kiyoshi
Bioscience, Biotechnology and Biochemistry, 2012 , vol. 76, # 2 p. 343 - 348
反应条件
1: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction 2: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction View Scheme
参考文献
Yamamoto, Yutaka; Kawashima, Daichi; Hashizume, Ayu; Hisamatsu, Makoto; Isono, Naoto
Bioscience, Biotechnology and Biochemistry, 2013 , vol. 77, # 9 p. 1949 - 1954
