sodium hydroxide; acylase I from Aspergillus melleus; cobalt(II) chloride in phosphate buffer T=39°C; pH=7.2; 48 h;

1: 93 percent / LiOH*H2O / H2O; ethanol / 1 h 2: aq. NaOH; acylase I from Aspergillus melleus / CoCl2*6H2O / aq. phosphate buffer / 48 h / 39 °C / pH 7.2 View Scheme

1: 98 percent / LiCl*H2O; water / dimethylsulfoxide / 16 h / 150 °C 2: 93 percent / LiOH*H2O / H2O; ethanol / 1 h 3: aq. NaOH; acylase I from Aspergillus melleus / CoCl2*6H2O / aq. phosphate buffer / 48 h / 39 °C / pH 7.2 View Scheme

1.1: H2SO4 / dioxane / 48 h / 20 °C 2.1: NaI / tetrahydrofuran / 16 h / Heating 3.1: NaH / dimethylformamide / 0.5 h 3.2: 93 percent / dimethylformamide / 4 h / 20 °C 4.1: 98 percent / LiCl*H2O; water / dimethylsulfoxide / 16 h / 150 °C 5.1: 93 percent / LiOH*H2O / H2O; ethanol / 1 h 6.1: aq. NaOH; acylase I from Aspergillus melleus / CoCl2*6H2O / aq. phosphate buffer / 48 h / 39 °C / pH 7.2 View Scheme

1.1: NaI / tetrahydrofuran / 16 h / Heating 2.1: NaH / dimethylformamide / 0.5 h 2.2: 93 percent / dimethylformamide / 4 h / 20 °C 3.1: 98 percent / LiCl*H2O; water / dimethylsulfoxide / 16 h / 150 °C 4.1: 93 percent / LiOH*H2O / H2O; ethanol / 1 h 5.1: aq. NaOH; acylase I from Aspergillus melleus / CoCl2*6H2O / aq. phosphate buffer / 48 h / 39 °C / pH 7.2 View Scheme

1.1: NaH / dimethylformamide / 0.5 h 1.2: 93 percent / dimethylformamide / 4 h / 20 °C 2.1: 98 percent / LiCl*H2O; water / dimethylsulfoxide / 16 h / 150 °C 3.1: 93 percent / LiOH*H2O / H2O; ethanol / 1 h 4.1: aq. NaOH; acylase I from Aspergillus melleus / CoCl2*6H2O / aq. phosphate buffer / 48 h / 39 °C / pH 7.2 View Scheme

sodium hydrogencarbonate in tetrahydrofuran 2 h;