Tris-HCl buffer; ethylenediaminetetraacetic acid; N-(N-L-γ-glutamyl-L-cysteinyl)glycine; nicotinamide adenine dinucleotide T=35°C; 6 h; investigation of enzyme, concentration, pH and buffer;

Sclerotinia sclerotiorum indolyl-3-acetaldoxime dehydratase T=23°C; pH=7.5; 1 h; aq. bufferDarknessEnzymatic reaction; Kinetics;

1.1: Sclerotinia sclerotiorum indolyl-3-acetaldoxime dehydratase / 1 h / 23 °C / pH 7.5 / aq. buffer; Darkness; Enzymatic reaction 2.1: diisobutylaluminium hydride / toluene / 0.33 h / -78 °C / Inert atmosphere 2.2: Inert atmosphere; Cooling with ice View Scheme

Tris-HCl buffer; ethylenediaminetetraacetic acid; N-(N-L-γ-glutamyl-L-cysteinyl)glycine; nicotinamide adenine dinucleotide T=35°C; 6 h; investigation of enzyme, concentration, pH and buffer;