1: With phosphoric acid trimethyl ester; N,N,N',N'-tetra-methylnaphthalene-1,8-diamine; trichlorophosphate T=0 - 4°C; 2: With triethylammonium hydrogen bicarbonate buffer T=20°C; pH=7.4 - 7.6; 1 h;

1.1: 93 percent / NaOCH3 / methanol / 3 h / 20 °C 2.1: (OCH3)3PO; POCl3; 1,8-bis(dimethylamino)naphthalene / 0 - 4 °C 2.2: aq. triethylammonium hydrogen bicarbonate buffer / 1 h / 20 °C / pH 7.4 - 7.6 View Scheme

Bacillus caldolyticus uracil phosphoribosyltransferase Condensation; 0.333333 h;

mit Hilfe eines Extraktes aus Escherichia coli (Stamm K-12);