trifluoroacetic acid in dichloromethane; chloroform; water

1: in water T=20°C; pH=7.6; 2 h; Enzymatic reaction; 2: With pyridoxal 5'-phosphate; Escherichia coli branched chain aminotransferase; Escherichia coli aspartate aminotransferase in sodium hydroxide T=20°C; pH=7.6; 15 h; Enzymatic reaction; Further stages.;

magnesium sulfate T=37°C; 1 h; Tris buffer (pH 9), leucine aminopeptidase;

1: 100 percent / aq. LiOH / tetrahydrofuran / 20 °C 2: 41 percent / cysteine sulfinic acid; acetaldehyde; aspartate aminotransferase / aq. NaOH / 20 °C / pH 7.6 View Scheme

1: 80 percent / aq. NaIO4; RuO2 / CCl4; acetonitrile / 24 h / 20 °C 2: 100 percent / aq. LiOH / tetrahydrofuran / 20 °C 3: 41 percent / cysteine sulfinic acid; acetaldehyde; aspartate aminotransferase / aq. NaOH / 20 °C / pH 7.6 View Scheme

1.1: 96 percent / H2SO4 / 12 h / Heating 2.1: LHMDS / tetrahydrofuran / 1 h / -78 °C 2.2: 73 percent / tetrahydrofuran / 2 h / -78 - -30 °C 3.1: 1N NaOH / Pseudomonas mendocina lipase / aq. phosphate buffer / 72 h / 4 °C / pH 7.0 / Enzymatic reaction 4.1: 100 percent / SOCl2 / Heating 5.1: 95 percent / TFA / CH2Cl2; H2O / 2 h / 20 °C / Acid hydrolysis 6.1: 98 percent / LiOH / H2O / pH 10 7.1: 78 percent / pyridoxal phosphate; cysteine sulfinic acid / Glutamic Oxalacetic Transaminase (GOT) / H2O / 24 h / 20 °C / pH 7 / Enzymatic reaction View Scheme

1.1: LHMDS / tetrahydrofuran / 1 h / -78 °C 1.2: 73 percent / tetrahydrofuran / 2 h / -78 - -30 °C 2.1: 1N NaOH / Pseudomonas mendocina lipase / aq. phosphate buffer / 72 h / 4 °C / pH 7.0 / Enzymatic reaction 3.1: 100 percent / SOCl2 / Heating 4.1: 95 percent / TFA / CH2Cl2; H2O / 2 h / 20 °C / Acid hydrolysis 5.1: 98 percent / LiOH / H2O / pH 10 6.1: 78 percent / pyridoxal phosphate; cysteine sulfinic acid / Glutamic Oxalacetic Transaminase (GOT) / H2O / 24 h / 20 °C / pH 7 / Enzymatic reaction View Scheme

1: 98 percent / LiOH / H2O / pH 10 2: 78 percent / pyridoxal phosphate; cysteine sulfinic acid / Glutamic Oxalacetic Transaminase (GOT) / H2O / 24 h / 20 °C / pH 7 / Enzymatic reaction View Scheme

1: 1N NaOH / Pseudomonas mendocina lipase / aq. phosphate buffer / 72 h / 4 °C / pH 7.0 / Enzymatic reaction 2: 100 percent / SOCl2 / Heating 3: 95 percent / TFA / CH2Cl2; H2O / 2 h / 20 °C / Acid hydrolysis 4: 98 percent / LiOH / H2O / pH 10 5: 78 percent / pyridoxal phosphate; cysteine sulfinic acid / Glutamic Oxalacetic Transaminase (GOT) / H2O / 24 h / 20 °C / pH 7 / Enzymatic reaction View Scheme

1: 100 percent / SOCl2 / Heating 2: 95 percent / TFA / CH2Cl2; H2O / 2 h / 20 °C / Acid hydrolysis 3: 98 percent / LiOH / H2O / pH 10 4: 78 percent / pyridoxal phosphate; cysteine sulfinic acid / Glutamic Oxalacetic Transaminase (GOT) / H2O / 24 h / 20 °C / pH 7 / Enzymatic reaction View Scheme