ammonium sulfate; Flavobacterium saccharophilum IFO 13984; magnesium sulfate in phosphate buffer Hydrolysis; pH=7.1; 96 h; Title compound not separated from byproducts;

anhydrous sodium benzoate; barium dihydroxide 1) DMF, 140 deg C, 120 h, 2) H2O, 80 deg C, 4 h; Yield given. Multistep reaction;

(i) NH3, MeOH, (ii) NaIO4, (iii) (hydrolysis); Multistep reaction;

1: 31.8 g / H2O; dioxane / 1.) 0-5 deg C, 1 h, 2.) room temp., 1 h 2: 5.1 g / pyridine / 15 h / Ambient temperature 3: 26.2 g / pyridine / 16 h / Ambient temperature 4: 16.9 g / sodium iodide / acetic anhydride / 0.5 h / 120 - 130 °C 5: 11.6 g / silver fluoride / pyridine / 3.5 h / 20 - 25 °C 6: 3.5 g / 5 M ammonium hydroxide / ethanol / 96 h / Ambient temperature 7: 2.4 g / bromine / methanol / 1 h / 0 - 5 °C 8: 104 mg / barium hydroxide / H2O / 5 h / 60 - 70 °C View Scheme