palladium 10 on activated carbon; hydrogen in methanol T=20°C; 10 h; Inert atmosphere;

1: 4-(N,N-dimethlyamino)pyridine; N-ethyl-N,N-diisopropylamine / tetrachloromethane; acetonitrile / 10 h / -10 °C / Inert atmosphere 2: palladium 10 on activated carbon; hydrogen / methanol / 10 h / 20 °C / Inert atmosphere View Scheme

1: toluene-4-sulfonic acid / toluene / 0.33 h / Reflux 2: 4-(N,N-dimethlyamino)pyridine; N-ethyl-N,N-diisopropylamine / tetrachloromethane; acetonitrile / 10 h / -10 °C / Inert atmosphere 3: palladium 10 on activated carbon; hydrogen / methanol / 10 h / 20 °C / Inert atmosphere View Scheme

Yersinia enterocolitica protein tyrosine phosphatase in dimethyl sulfoxide T=20°C; pH=7.1; Enzyme kinetics;