sodium hydroxide; potassium dihydrogenphosphate; pig liver esterase T=27°C; pH 7.8;

1: 95 percent / conc. H2SO4 / 4 h / 150 °C 2: 75 percent / 0.1 M KH2PO4, 1 N NaOH / pig liver esterase / 27 °C / pH 7.8 View Scheme

acetyl chloride Esterification; unreacted MeOH removing; residue washing with 0.5 M NaOH to extract product into aqueous phase; treatment with 0.5 M HCl;