hydrogen; palladium dihydroxide in methanol P=2280 Torr; Yield given;

hydrogenchloride 5 h; Heating;

1: 49 percent / Aspergillus species protease; potassium phosphate buffer; aq. NaOH / acetonitrile / 24 h / 23 °C / pH 7 2: 45 percent / aq. HCl / 5 h / Heating View Scheme

1: 94 percent / Et3N / CH2Cl2 / 20 °C 2: 49 percent / Aspergillus species protease; potassium phosphate buffer; aq. NaOH / acetonitrile / 24 h / 23 °C / pH 7 3: 45 percent / aq. HCl / 5 h / Heating View Scheme

2-(2,4,6-trimethyl-phenyl)-2,5,6,7-tetrahydro-pyrrolo[2,1-c] [1,2,4]triazol-4-ylium perchlorate; (4aR,9aS)-4-hydroxy-4,4a,9,9a-tetrahydroindeno[2,1-b][1,4]oxazin-3(2H)-one; 1,8-diazabicyclo[5.4.0]undec-7-ene in dichloromethane T=23°C; 18 h; Resolution of racemate;