dihydroxyacetone phosphate; ethylenediaminetetraacetic acid; 1,4-dithio-DL-threitol; nicotinamide adenine dinucleotide; trisodium phosphorothioate; 2-oxo-propionic acid; magnesium chloride; phosphoglycerate kinase; immobil. triosephosphate isomerase; glyceraldehyde-3-phosphate dehydrogenase; lactate dehydrogenase in water 72 h; Ambient temperature1) pH 7.5;

1: 98 percent / DHA / immobilized Glycerokinase / 2 h / Ambient temperature 2: 80 percent / 1) NAD, DTT, pyruvate, MgCl2, EDTA, Dihydroxyacetone Phosphate, 2) trisodium monothiophosphate / 1) immobil. triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, lactate dehydrogenase / H2O / 72 h / Ambient temperature; 1) pH 7.5 View Scheme

tri-(hydroxymethyl)-methylamine; magnesium chloride; nucleotide 5'-diphosphate kinase in water T=30°C; Substitution; 1 h; Enzymatic reaction;