bovine testes β-galactosidase; McIlvain buffer in water T=37°C; pH=4.3; 50 h;

1: 57.5 percent / mercuric cyanide / benzene; nitromethane / 72 h / Ambient temperature 2: 81.4 percent / M sodium methoxide / methanol / 5 h / Ambient temperature 3: 81.9 percent / zinc chloride / Ambient temperature 4: 78.9 percent / pyridine / 48 h / Ambient temperature 5: 85.5 percent / 60percent aq. acetic acid / 0.67 h / 98 °C 6: sodium methoxide / methanol View Scheme

1: 57.5 percent / mercuric cyanide / benzene; nitromethane / 72 h / Ambient temperature 2: 81.4 percent / M sodium methoxide / methanol / 5 h / Ambient temperature 3: 81.9 percent / zinc chloride / Ambient temperature 4: 78.9 percent / pyridine / 48 h / Ambient temperature 5: 85.5 percent / 60percent aq. acetic acid / 0.67 h / 98 °C 6: sodium methoxide / methanol View Scheme

sodium salt of phosphorous acid; β-galactosidase in water; N,N-dimethyl-formamide T=37°C; 3 h; effect of donor/acceptor ratio, also immobilized β-galactosidase; Product distribution;

sodium methylate in methanol

1: 81.9 percent / zinc chloride / Ambient temperature 2: 78.9 percent / pyridine / 48 h / Ambient temperature 3: 85.5 percent / 60percent aq. acetic acid / 0.67 h / 98 °C 4: sodium methoxide / methanol View Scheme

1: 85.5 percent / 60percent aq. acetic acid / 0.67 h / 98 °C 2: sodium methoxide / methanol View Scheme

1: 78.9 percent / pyridine / 48 h / Ambient temperature 2: 85.5 percent / 60percent aq. acetic acid / 0.67 h / 98 °C 3: sodium methoxide / methanol View Scheme

1: 81.4 percent / M sodium methoxide / methanol / 5 h / Ambient temperature 2: 81.9 percent / zinc chloride / Ambient temperature 3: 78.9 percent / pyridine / 48 h / Ambient temperature 4: 85.5 percent / 60percent aq. acetic acid / 0.67 h / 98 °C 5: sodium methoxide / methanol View Scheme

Phosphoenolpyruvate; CgtB galactosyltransferase N26K, L68I, K151E, L227G, E234D mutant; potassium chloride; Reduced nicotinamide-adenine dinucleotide; magnesium chloride; manganese (II) chloride; BSA T=37°C; pH=7.5; aq. bufferEnzymatic reaction;