phosphodiesterase I in water T=37°C; 6 h; pH 8.8;

hydrogenchloride T=35 - 40°C; time 30 - 60 min;

1: N,N'-dicyclohexylcarbodiimide / ethyl acetate / 12 h / 4 °C 2: 84 percent / dimethylformamide / 12 h / 25 °C 3: 85 percent / alkaline mesintericopeptidase / H2O / 6 h / 25 °C / pH 8.0 4: 100 percent / phosphodiesterase I / H2O / 6 h / 37 °C / pH 8.8 View Scheme

1: 84 percent / dimethylformamide / 12 h / 25 °C 2: 85 percent / alkaline mesintericopeptidase / H2O / 6 h / 25 °C / pH 8.0 3: 100 percent / phosphodiesterase I / H2O / 6 h / 37 °C / pH 8.8 View Scheme

1: 85 percent / alkaline mesintericopeptidase / H2O / 6 h / 25 °C / pH 8.0 2: 100 percent / phosphodiesterase I / H2O / 6 h / 37 °C / pH 8.8 View Scheme