1: 59 percent / aq acetic acid (80percent) / 20 h / 60 °C 2: 55.7 percent / imidazole / CHCl3 / 40 h / Heating 3: 74.2 percent / silver trifluoromethanesulphonate, 1,1,3,3-tetramethylurea / CH2Cl2 / 4.5 h / Ambient temperature 4: 1) sodium, 2) ammonium chloride / 1) liq NH3, THF, -70 deg C, 4 h 20 min, 2) room temp., 4.5 h View Scheme

recombinant His6-tagged validamycin glycosyltransferase T=30°C; pH=7.5; aq. bufferEnzymatic reaction;

sodium methylate in methanol 5 h; Ambient temperature;

1: 1)methanolic sodium methoxyde (1M), 2) sodium hydride / 1) methanol, room temp., 50 min, 2) DMF, room temp., 23 h 2: 59 percent / aq acetic acid (80percent) / 20 h / 60 °C 3: 55.7 percent / imidazole / CHCl3 / 40 h / Heating 4: 74.2 percent / silver trifluoromethanesulphonate, 1,1,3,3-tetramethylurea / CH2Cl2 / 4.5 h / Ambient temperature 5: 1) sodium, 2) ammonium chloride / 1) liq NH3, THF, -70 deg C, 4 h 20 min, 2) room temp., 4.5 h View Scheme

ammonium chloride; sodium 1) liq NH3, THF, -70 deg C, 4 h 20 min, 2) room temp., 4.5 h; Multistep reaction;

ammonium sulfate; Flavobacterium saccharophilum IFO 13984; magnesium sulfate in phosphate buffer Hydrolysis; pH=7.1; 96 h; Title compound not separated from byproducts;

ammonium sulfate; Flavobacterium saccharophilum IFO 13984; magnesium sulfate in phosphate buffer Hydrolysis; pH=7.1; 96 h; Title compound not separated from byproducts;

VldW in aq. phosphate buffer pH=7.2; Enzymatic reaction; Kinetics; regioselective reaction;