water; trifluoroacetic acid in dichloromethane T=20°C; 0.166667 h;

potassium hydroxide; Bacillus stearothermophilus pyrimidine nucleoside phosphorylase; tris(hydroxymethyl)methylamine hydrochloride in water T=37°C; pH=7.5; aq. bufferEnzymatic reaction;

trifluoroacetic acid Ambient temperature;