1: 96 percent / pyridine / CH2Cl2 2: HF; pyridine / CH2Cl2 / 0 °C 3: 90 percent / pyridine / 0 °C View Scheme

Stage #1: <a href="#" onclick="return RXDsubstances(event, this, '3572919', constants.ctx, '0')">3',5'-O-DTBS-guanosine</a> <b>With</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '103853', constants.ctx, '0')">1H-imidazole</a>&nbsp;<b>in</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '605365', constants.ctx, '0')">N,N-dimethyl-formamide</a> <br />T=0 - 25&deg;C;&nbsp;1&nbsp;h;&nbsp;<br />Stage #2: <a href="#" onclick="return RXDsubstances(event, this, '505999', constants.ctx, '0')">tert-butyldimethylsilyl chloride</a> T=60&deg;C;&nbsp;2&nbsp;h;&nbsp;<br /> &nbsp;

<b>Multi-step reaction with 2 steps<br /> </b>1: imidazole / dimethylformamide / 0 &deg;C<br />2: imidazole / dimethylformamide / 60 &deg;C<br /> <br /> &nbsp;