hydrogen; palladium on activated charcoal in ethyl acetate

sodium azide; Arthrobacter sp. halohydrin dehalogenase in dimethyl sulfoxide T=20°C; pH=7; 1.5 h; aq. bufferEnzymatic reaction; regioselective reaction;

tris-(2-chloro-ethyl)-amine; triethylamine in toluene; benzene T=-70 - 20°C;

1.1: sodium hydride / dimethyl sulfoxide; mineral oil / 0.5 h / 0 - 20 °C / Inert atmosphere 1.2: 20 h / Inert atmosphere 2.1: sodium azide; Arthrobacter sp. halohydrin dehalogenase / dimethyl sulfoxide / 1.5 h / 20 °C / pH 7 / aq. buffer; Enzymatic reaction View Scheme