1.1: hydrogenchloride; water / 6 h / 20 °C 2.1: sodium amalgam; potassium hydroxide / 0.08 h / 80 °C / Darkness 3.1: recombinant oxygen-independent coproporphyrinogen III oxidase; [14C]-Ademetionine; Reduced nicotinamide-adenine dinucleotide; threo-1,4-dimercapto-2,3-butanediol / 1 h / 37 °C / pH 7 / Inert atmosphere; Bis-Tris buffer; Enzymatic reaction 3.2: 0.25 h / Bis-Tris buffer View Scheme