phosphate buffer; nitrilase I in methanol T=37°C; pH=8;

1.1: acetone cyanohydrin / DIEA / 0.75 h / 0 °C 1.2: H2SO4 / -20 °C 2.1: 68 percent / nitrilase I; phosphate buffer / methanol / 37 °C / pH 8 View Scheme

nitrosyl sulfuric acid in N,N-dimethyl-formamide T=0°C; 0.5 h; Title compound not separated from byproducts;

1.1: 1.69 g / SnBr2 / CH2Cl2 / 20 °C 2.1: 88 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; ethanol; hexane / 24 h / 30 °C / pH 6.0 3.1: 35 percent / nitrosylsulfuric acid / dimethylformamide / 24 h / 20 °C 4.1: SnBr2 / CH2Cl2 / 20 °C 4.2: 92 percent / CH2Cl2 / 20 °C 5.1: 45 percent / immobilized Burkholderia cepacia lipase; phosphate buffer; NaOH / decane; H2O / 38 h / 30 °C / pH 7.0 6.1: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; dimethylformamide; hexane / 24 h / 30 °C / pH 6.0 7.1: nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 °C View Scheme

1: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; dimethylformamide; hexane / 24 h / 30 °C / pH 6.0 2: nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 °C View Scheme

1.1: 88 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; ethanol; hexane / 24 h / 30 °C / pH 6.0 2.1: 35 percent / nitrosylsulfuric acid / dimethylformamide / 24 h / 20 °C 3.1: SnBr2 / CH2Cl2 / 20 °C 3.2: 92 percent / CH2Cl2 / 20 °C 4.1: 45 percent / immobilized Burkholderia cepacia lipase; phosphate buffer; NaOH / decane; H2O / 38 h / 30 °C / pH 7.0 5.1: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; dimethylformamide; hexane / 24 h / 30 °C / pH 6.0 6.1: nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 °C View Scheme

1: 45 percent / immobilized Burkholderia cepacia lipase; phosphate buffer; NaOH / decane; H2O / 38 h / 30 °C / pH 7.0 2: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; dimethylformamide; hexane / 24 h / 30 °C / pH 6.0 3: nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 °C View Scheme

1.1: 35 percent / nitrosylsulfuric acid / dimethylformamide / 24 h / 20 °C 2.1: SnBr2 / CH2Cl2 / 20 °C 2.2: 92 percent / CH2Cl2 / 20 °C 3.1: 45 percent / immobilized Burkholderia cepacia lipase; phosphate buffer; NaOH / decane; H2O / 38 h / 30 °C / pH 7.0 4.1: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; dimethylformamide; hexane / 24 h / 30 °C / pH 6.0 5.1: nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 °C View Scheme

1: 60 percent / aq. NaOH / 0.5 h / 55 °C 2: (R)-1-phenylethylamine / ethanol / 0.5 h / Heating View Scheme

1: 85 percent / Cl2 / acetic acid / 20 h / 55 °C 2: 60 percent / aq. NaOH / 0.5 h / 55 °C 3: (R)-1-phenylethylamine / ethanol / 0.5 h / Heating View Scheme