4-硝基苯基-Β-D-乳糖苷合成路线 （共 15 条）
反应条件
1: boron trifluoride diethyl etherate / dichloromethane / 0 - 20 °C / |Inert atmosphere 2: methanol; sodium methylate / 20 °C / pH 10 - 11 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
Wei, Jinhua; Lv, Xun; Lue, Yang; Yang, Gangzhu; Fu, Lifeng; Yang, Liu; Wang, Jianjun; Gao, Jianhui; Cheng, Shuihong; Duan, Qian; Jin, Cheng; Li, Xuebing
European Journal of Organic Chemistry, 2013 , # 12 p. 2414 - 2419
反应条件
1: boron trifluoride diethyl etherate / dichloromethane / 0 - 20 °C / |Inert atmosphere 2: methanol; sodium methylate / 20 °C / pH 10 - 11 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
Wei, Jinhua; Lv, Xun; Lue, Yang; Yang, Gangzhu; Fu, Lifeng; Yang, Liu; Wang, Jianjun; Gao, Jianhui; Cheng, Shuihong; Duan, Qian; Jin, Cheng; Li, Xuebing
European Journal of Organic Chemistry, 2013 , # 12 p. 2414 - 2419
反应条件
β-D-galactosidase from Bacillus circulans in water; acetonitrile 1.) phosphate buffer pH 7.0, 40 deg C, 6 h, 2.) 95 deg C, 10 min.; Yield given. Yields of byproduct given;
参考文献
Murata; Akimoto; Horimoto; Usui
Bioscience, biotechnology, and biochemistry, 1997 , vol. 61, # 7 p. 1118 - 1120
反应条件
β4galT; manganese (II) chloride; bovine serum albumin in various solvent(s) T=37°C; pH=7.5; Enzymatic reaction; Product distribution; Further Variations:Reagents;
参考文献
Blixt; Brown; Schur; Wakarchuk; Paulson
Journal of Organic Chemistry, 2001 , vol. 66, # 7 p. 2442 - 2448
反应条件
hydrogen; palladium dihydroxide in water T=20°C; 15 h; atmospheric pressure;
参考文献
Tetrahedron, , vol. 61, # 24 p. 5895 - 5905
