1.1: LDA / tetrahydrofuran 1.2: HMPA / tetrahydrofuran 2.1: TiCl4; Et3N; (Me3Si)2NH 3.1: NaCNBH3 / CH2Cl2 4.1: aq. NBS / acetonitrile View Scheme

pyruvate dehydrogenase; N-terminal murine erythroid 5-aminolevulinate synthase/C-terminal Escherichia coli 8-amino-7-oxononanoate synthase chimeric protein; nicotinamide adenine dinucleotide; 2-oxo-propionic acid; magnesium chloride T=30°C; pH=7.5; aq. bufferEnzymatic reaction; Kinetics;