nitrosyl sulfuric acid in N,N-dimethyl-formamide T=20°C; 24 h; Title compound not separated from byproducts;

1.1: 35 percent / nitrosylsulfuric acid / dimethylformamide / 24 h / 20 °C 2.1: SnBr2 / CH2Cl2 / 20 °C 2.2: 92 percent / CH2Cl2 / 20 °C 3.1: 45 percent / immobilized Burkholderia cepacia lipase; phosphate buffer; NaOH / decane; H2O / 38 h / 30 °C / pH 7.0 4.1: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; dimethylformamide; hexane / 24 h / 30 °C / pH 6.0 5.1: nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 °C 6.1: 13.0 mg / CH2Cl2 7.1: 48 percent / silica gel / diethyl ether 8.1: 100 percent / Krebsiella oxytoca esterase; phosphate buffer / H2O / 39 °C / pH 7.0 View Scheme

1.1: 35 percent / nitrosylsulfuric acid / dimethylformamide / 24 h / 20 °C 2.1: SnBr2 / CH2Cl2 / 20 °C 2.2: 92 percent / CH2Cl2 / 20 °C 3.1: 45 percent / immobilized Burkholderia cepacia lipase; phosphate buffer; NaOH / decane; H2O / 38 h / 30 °C / pH 7.0 4.1: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H2O; dimethylformamide; hexane / 24 h / 30 °C / pH 6.0 5.1: nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 °C View Scheme

<b>Multi-step reaction with 6 steps<br /> </b>1: 45 percent / immobilized Burkholderia cepacia lipase; phosphate buffer; NaOH / decane; H₂O / 38 h / 30 &deg;C / pH 7.0<br />2: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H₂O; dimethylformamide; hexane / 24 h / 30 &deg;C / pH 6.0<br />3: 6 percent / nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 &deg;C<br />4: Amberlyst-15 H⁺ form / 0.17 h<br />5: 1.69 g / SnBr₂ / CH₂Cl₂ / 20 &deg;C<br />6: 88 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H₂O; ethanol; hexane / 24 h / 30 &deg;C / pH 6.0<br /> <br /> &nbsp;

<b>Multi-step reaction with 5 steps<br /> </b>1: 75 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H₂O; dimethylformamide; hexane / 24 h / 30 &deg;C / pH 6.0<br />2: 6 percent / nitrosylsulfuric acid / dimethylformamide / 0.5 h / 0 &deg;C<br />3: Amberlyst-15 H⁺ form / 0.17 h<br />4: 1.69 g / SnBr₂ / CH₂Cl₂ / 20 &deg;C<br />5: 88 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H₂O; ethanol; hexane / 24 h / 30 &deg;C / pH 6.0<br /> <br /> &nbsp;

<b>Multi-step reaction with 3 steps<br /> </b>1: Amberlyst-15 H⁺ form / 0.17 h<br />2: 1.69 g / SnBr₂ / CH₂Cl₂ / 20 &deg;C<br />3: 88 percent / Rhodococcus rhodochrous IFO 15564; phosphate buffer / H₂O; ethanol; hexane / 24 h / 30 &deg;C / pH 6.0<br /> <br /> &nbsp;