1: With triethylamine in dichloromethane; water T=-40 - 0°C; 3 h; 2: With hydrogenchloride in dichloromethane; water 1 h; 3: With sodium hydroxide in dichloromethane; water T=0°C; pH=3.2; 2 h;

1: 7-Aminodesacetoxycephalosporanic acid With 1H-imidazole; chloro-trimethyl-silane in dichloromethane 3 - 4 h; Heating / reflux; 2: C16H19NO7 in DMF (N,N-dimethyl-formamide); dichloromethane T=-45 - -20°C; 2.5 h; 3: With hydrogenchloride in DMF (N,N-dimethyl-formamide); dichloromethane; water T=0 - 5°C; 0.25 h;

formic acid; ammonia; immobilized Pen-G acylase mutant Phe-24-Ala in water T=12°C; pH=7.3; 2.5 h; Enzymatic reaction; Product distribution / selectivity;

immobilised Pen G acylase in water Enzymatic reaction;

disodium hydrogenphosphate in water T=60°C; 1 h; pH 9.1;

acid