potassium hydroxide in water 0.333333 h; Ambient temperature;

β-D-glucopyranose; hesperidinase; McIlvaine buffer; butan-1-ol in dimethyl sulfoxide 1.) 40 deg C, 5 min; 2.) reflux, 20 min;

1: 32 mg / 1.) hesperidinase/ citrate-phosphate; 2.) n-BuOH / dimethylsulfoxide / 1.) pH=4.6, 74 deg C, 20 min; 2.) reflux, 20 min 2: 24 mg / 1) hesperidinase, 0.05 M McIlvaine buffer, 4percent β-D-glucose; 2.) n-BuOH / dimethylsulfoxide / 1.) 40 deg C, 5 min; 2.) reflux, 20 min View Scheme

acetic acid

UDP glucose:flavonol glucosyltransferase enzyme preparation from Norway spruce needles in water 0.75 h; pH 8.0 bicine buffer;

T=30°C; 336 h; cell suspension culture of Vitis hybrid; Yield given. Further byproducts given. Yields of byproduct given;

hydrogenchloride; water T=100°C; 1 h;

hydrogenchloride; water T=90°C; 1 h;