MOPS buffer; calcium chloride; bovine trypsin in dimethyl sulfoxide T=25°C; 5 h;

benzotriazol-1-ol; triethylamine; dicyclohexyl-carbodiimide in N,N-dimethyl-formamide Ambient temperature;

1: 92 percent / 4-dimethylaminopyridine, N,N'-dicyclohexylcarbodiimide / dioxane; CH2Cl2 / a) 0 deg C, 1 h, b) RT, 12 h 2: 96 percent / H2 / 10 percent Pd/C / ethanol; diethyl ether / Ambient temperature 3: 88 percent / 3-morpholino-1-propanesulfonate, CaCl2 (pH 8.0) / dimethylsulfoxide / 12 h / 25 °C / trypsin View Scheme

1: 85 percent / H2 / 10 percent Pd/C / ethanol; diethyl ether / Ambient temperature 2: 75 percent / 3-morpholino-1-propanesulfonate, CaCl2 (pH 8.0) / dimethylsulfoxide / 12 h / 25 °C / trypsin View Scheme

1: 98 percent / H2 / 10 percent Pd/C / ethanol; diethyl ether / Ambient temperature 2: 72 percent / 3-morpholino-1-propanesulfonate, CaCl2 (pH 8.0) / dimethylsulfoxide / 2 h / 25 °C / trypsin View Scheme

1: 96 percent / H2 / 10 percent Pd/C / ethanol; diethyl ether / Ambient temperature 2: 88 percent / 3-morpholino-1-propanesulfonate, CaCl2 (pH 8.0) / dimethylsulfoxide / 12 h / 25 °C / trypsin View Scheme

1: 94 percent / H2 / 10 percent Pd/C / ethanol; diethyl ether / Ambient temperature 2: 79 percent / 3-morpholino-1-propanesulfonate, CaCl2 (pH 8.0) / dimethylsulfoxide / 1 h / 25 °C / trypsin View Scheme

1: 1.) H2 / 1.) 10percent Pd/C / 2.) EtOH, Et2O 2: 95 percent / bovine trypsin, MOPS buffer (pH 8.0), CaCl2 / dimethylsulfoxide / 5 h / 25 °C View Scheme

4-morpholinepropanesulfonate buffer; methyltrypsin in water; dimethyl sulfoxide T=25°C; 4 h;

Streptomyces griseus trypsin in dimethyl sulfoxide T=25°C; pH=8.0; 24 h;