recombinant human cytochrome P450 or CYP enzymes; all-trans-vitamin A acid hydroxylation; Enzymatic reaction; Enzyme kinetics; Further Variations:Reagents;
参考文献
Honda Malca, Sumire; Scheps, Daniel; Kuehnel, Lisa; Venegas-Venegas, Elena; Seifert, Alexander; Nestl, Bettina M.; Hauer, Bernhard
Chemical Communications, 2012 , vol. 48, # 42 p. 5115 - 5117
putidaredoxin reducatse; wild type; putidaredoxin in phosphate buffer T=37°C; pH=7.0; Enzyme kineticsProduct distribution; Further Variations:enzymes;
参考文献
Koo, Laura S.; Immoos, Chad E.; Cohen, Michael S.; Farmer, Patrick J.; Ortiz de Montellano, Paul R.
Journal of the American Chemical Society, 2002 , vol. 124, # 20 p. 5684 - 5691
Cameron, Andrew G.; Knight, David W.
Journal of the Chemical Society, Perkin Transactions 1: Organic and Bio-Organic Chemistry (1972-1999), 1986 , p. 161 - 168
1: 86 percent / CHCl3 / 48 h / Heating 2: 92 percent / sodium borohydride / ethanol / 0.25 h / -30 °C 3: 10percent methanolic hydroxide View Scheme
参考文献
Cameron, Andrew G.; Knight, David W.
Journal of the Chemical Society, Perkin Transactions 1: Organic and Bio-Organic Chemistry (1972-1999), 1986 , p. 161 - 168
1: 92 percent / sodium borohydride / ethanol / 0.25 h / -30 °C 2: 10percent methanolic hydroxide View Scheme
参考文献
Cameron, Andrew G.; Knight, David W.
Journal of the Chemical Society, Perkin Transactions 1: Organic and Bio-Organic Chemistry (1972-1999), 1986 , p. 161 - 168
