Tris-HCl buffer; coenzyme-A; rat liver homogenate; adenosine 5'-triphosphoric acid; magnesium chloride; nicotinamide adenine dinucleotide in methanol T=37°C; 1 h; incubation aerobically; pH= 8.5;

Multi-step reaction with 5 steps 1: H2SO4 2: 420 mg / pyridine / benzene / 18 h / Ambient temperature3: 340 mg / CrO3, aq. H2SO4 / acetone / 0.5 h / Ambient temperature4: 230 mg / acetic acid / 18 h / Ambient temperature5: 1.) NaBH4, CH3COOH, 2.) 10percent aq. NaOH / 1.) a) 50 deg C, 1 h, b) RT, 6 h, 2.) methanol, reflux, 3 h