Pseudomonas aeruginosa lipase Met16Ala/Leu17Phe/Leu162Asn mutant T=25°C; pH=7.5; aq. phosphate bufferEnzymatic reaction; Kinetics; Reagent/catalyst;

Tris-HCl buffer; N,N-dimethyl-formamide; calcium chloride T=25°C; with chymotrypsin, pH 8; dissociation constant of enzyme-substrate complex KS; k(S/R); Rate constantEquilibrium constant;