β-D-galactosidase from B. circulans ATCC 31382; sodium acetate in water T=50°C; Etherification; pH=5.5; 9 h;

1.1: pyridine / 50 °C 2.1: acetyl chloride / methanol / 0 - 20 °C / |Inert atmosphere 3.1: potassium carbonate; tetra(n-butyl)ammonium hydrogensulfate / water; dichloromethane / 20 °C 4.1: sodium methylate / methanol 4.2: Amberlite IR-120 (H+) resin View Scheme

1.1: potassium carbonate; tetra(n-butyl)ammonium hydrogensulfate / water; dichloromethane / 20 °C 2.1: sodium methylate / methanol 2.2: Amberlite IR-120 (H+) resin View Scheme

recombinant glutathione S-transferase-fused Escherichia coli O55:H7 β1,3-galactosyltransferase; manganese (II) chloride T=20°C; pH=7; 48 h; HEPES bufferEnzymatic reaction;

β-galactosidase in acetonitrile Enzymatic reaction;

lacto-N-biosidase from Bifidobacterium bifidum JCM1254 in aq. buffer T=30°C; pH=4.5; Enzymatic reaction; KineticsMechanism;