β-D-galactosidase from B. circulans ATCC 31382; sodium acetate in water T=50°C; Etherification; pH=5.5; 3.5 h;

β-galactosidase from bovine testes; sodium phosphate-citrate buffer T=37°C; 48 h; pH 4.3;

1.1: sodium methylate / methanol / 0.5 h / 20 °C / pH 10 1.2: Dowex H+ 2.1: toluene-4-sulfonic acid / N,N-dimethyl-formamide; acetonitrile / 1 h / 20 °C 3.1: dichloromethane / 0.33 h / Inert atmosphere; Molecular sieve 3.2: 2 h / -20 °C / Inert atmosphere; Molecular sieve 4.1: water; acetic acid / dichloromethane / 20 °C 5.1: pyridine / 20 °C 6.1: sodium methylate / methanol / 0.5 h 6.2: 1 h 6.3: Dowex H+ View Scheme

1.1: trimethylsilyl trifluoromethanesulfonate / dichloromethane / -20 °C 2.1: sodium methylate / methanol / 0.5 h / 20 °C / pH 10 2.2: Dowex H+ 3.1: toluene-4-sulfonic acid / N,N-dimethyl-formamide; acetonitrile / 1 h / 20 °C 4.1: dichloromethane / 0.33 h / Inert atmosphere; Molecular sieve 4.2: 2 h / -20 °C / Inert atmosphere; Molecular sieve 5.1: water; acetic acid / dichloromethane / 20 °C 6.1: pyridine / 20 °C 7.1: sodium methylate / methanol / 0.5 h 7.2: 1 h 7.3: Dowex H+ View Scheme

1.1: water; acetic acid / dichloromethane / 20 °C 2.1: pyridine / 20 °C 3.1: sodium methylate / methanol / 0.5 h 3.2: 1 h 3.3: Dowex H+ View Scheme

1.1: pyridine / 20 °C 2.1: sodium methylate / methanol / 0.5 h 2.2: 1 h 2.3: Dowex H+ View Scheme

1.1: toluene-4-sulfonic acid / N,N-dimethyl-formamide; acetonitrile / 1 h / 20 °C 2.1: dichloromethane / 0.33 h / Inert atmosphere; Molecular sieve 2.2: 2 h / -20 °C / Inert atmosphere; Molecular sieve 3.1: water; acetic acid / dichloromethane / 20 °C 4.1: pyridine / 20 °C 5.1: sodium methylate / methanol / 0.5 h 5.2: 1 h 5.3: Dowex H+ View Scheme

1.1: dichloromethane / 0.33 h / Inert atmosphere; Molecular sieve 1.2: 2 h / -20 °C / Inert atmosphere; Molecular sieve 2.1: water; acetic acid / dichloromethane / 20 °C 3.1: pyridine / 20 °C 4.1: sodium methylate / methanol / 0.5 h 4.2: 1 h 4.3: Dowex H+ View Scheme

1.1: trimethylsilyl trifluoromethanesulfonate / dichloromethane / -20 °C 2.1: sodium methylate / methanol / 0.5 h / 20 °C / pH 10 2.2: Dowex H+ 3.1: toluene-4-sulfonic acid / N,N-dimethyl-formamide; acetonitrile / 1 h / 20 °C 4.1: dichloromethane / 0.33 h / Inert atmosphere; Molecular sieve 4.2: 2 h / -20 °C / Inert atmosphere; Molecular sieve 5.1: water; acetic acid / dichloromethane / 20 °C 6.1: pyridine / 20 °C 7.1: sodium methylate / methanol / 0.5 h 7.2: 1 h 7.3: Dowex H+ View Scheme

Bacillus circulans T=25°C; 5 h; Enzymatic reaction; Kinetics;