aqualisin I; calcium chloride; 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid in water T=40°C; also with Proteinase K, Subtilisin Carlsberg and Subtilisin BPN'; Rate constant;

endopeptidase in various solvent(s) T=25°C; 1 h; pH=8.0; apparent rate of hydrolysis;