1: 91.7 percent / acetic anhydride / 3 h 2: 76.1 percent / SOCl2 / 0.67 h / 40 °C 3: 82.6 percent / AcONa, acetic anhydride / 3 h 4: 62.5 percent / ethanol / 16 h / Heating 5: 1 M aq. NaOH / acetone / 1 h / -20 - -15 °C 6: 0.5 M H2SO4 / acetone / 0.5 h / Ambient temperature 7: 5 M H2SO4 / 6 h / 45 - 47 °C View Scheme

Tris-HCl buffer; N74D Escherichia coli asparagine synthetase B mutant; 2-bis(2-hydroxyethyl)-amino-2-(hydroxymethyl)-1,3-propanediol; magnesium chloride T=37°C; Hydrolysis; pH=6.5; 0.333333 h; Enzyme kinetics; Further Variations:ReagentspH-values;