sodium hydroxide T=5°C; 12 h;

1: 72.5 percent / N,N'-dicyclohexylcarbodiimide / ethyl acetate / 24 h / Ambient temperature 2: phosphodiesterase I / 6 h / 37 °C / pH = 8.8 3: 84.6 percent / 2 N sodium hydroxide / 12 h / 5 °C View Scheme

1: 72.5 percent / N,N'-dicyclohexylcarbodiimide / ethyl acetate / 24 h / Ambient temperature 2: phosphodiesterase I / 6 h / 37 °C / pH = 8.8 3: 84.6 percent / 2 N sodium hydroxide / 12 h / 5 °C View Scheme

1: phosphodiesterase I / 6 h / 37 °C / pH = 8.8 2: 84.6 percent / 2 N sodium hydroxide / 12 h / 5 °C View Scheme