(6R)-5-乙酰氨基-3,5-二脱氧-6-[(1R,2R)-1,2,3-三羟基丙基]-beta-L-苏式-己-2-酮吡喃糖基-(2->6)-beta-D-吡喃半乳糖基-(1->4)-2-乙酰氨基-2-脱氧-beta-D-吡喃葡萄糖基-(1->3)-beta-D-吡喃半乳糖基-(1->4)-D-吡喃葡萄糖合成路线 (共 3 条)
反应条件
Galβ1,4GlcNAcα2,6 sialyltransferase; Alkarsodyl; bovine serum albumin
T=37°C; 22 h; pH=6.5; Yield given;
参考文献
Sabesan,S.; Paulson,J.C.
Journal of the American Chemical Society, 1986 , vol. 108, p. 2068
反应条件
1: bovine serum albumin, 0.1M sodium cacodylate, 0.1M manganese chloride, galactosyltransferase / 6 h / 37 °C / pH=7.0
2: 0.1M sodium cacodylate, bovine serum albumin, Galβ1,4GlcNAcα2,6 sialyltransferase / 22 h / 37 °C / pH=6.5
View Scheme
参考文献
Sabesan,S.; Paulson,J.C.
Journal of the American Chemical Society, 1986 , vol. 108, p. 2068
反应条件
1: bovine serum albumin, 0.1M sodium cacodylate, 0.1M manganese chloride, galactosyltransferase / 6 h / 37 °C / pH=7.0
2: 0.1M sodium cacodylate, bovine serum albumin, Galβ1,4GlcNAcα2,6 sialyltransferase / 22 h / 37 °C / pH=6.5
View Scheme
参考文献
Sabesan,S.; Paulson,J.C.
Journal of the American Chemical Society, 1986 , vol. 108, p. 2068