potassium phosphate buffer; lyophilized Zea mays nitrilase ZmNIT2 EC 3.5.5.1 in water T=30°C; pH=7.15; 14 h; Title compound not separated from byproducts;
1: 3-phenylpropiononitrile With n-butyllithium in hexane Inert atmosphere; 2: carbon dioxide in hexane T=-78°C; Inert atmosphere; 3: With hydrogenchloride in water pH=2;
参考文献
Recio III, Antonio; Tunge, Jon A.
Organic Letters, 2009 , vol. 11, # 24 p. 5630 - 5633
