1: 1) cobalt acetate, 25percent aq. NH4OH, 2) TFA / 1) acylase I, H2O, 38 deg C, 2) 50 deg C, 10 min 2: 82 percent / 1M NaOH / H2O; dioxane / 1) 5 deg C, 10 min, 2) RT, 1h View Scheme

1: Behandeln in gepufferter wss. Loesung (pH 4,5) in Gegenwart eines Papain-Praeparats 2: aqueous hydrochloric acid View Scheme

ammonium hydroxide; anhydrous cobalt diacetate; trifluoroacetic acid 1) acylase I, H2O, 38 deg C, 2) 50 deg C, 10 min; Yield given. Multistep reaction;