post-proline endopeptidase in water; dimethyl sulfoxide T=37°C; 0.5 h; pH 7.5; Mechanism;

post-proline endopeptidase in water; dimethyl sulfoxide T=37°C; 0.5 h; pH 7.5; Mechanism;

1: tetrahydrofuran / 1.) 3 h, 0 deg C; 2.) 12 h, r.t. 2: HBr / acetic acid / 0.17 h / Ambient temperature 3: N-ethylpiperidine / dimethylformamide / 12 h / Ambient temperature View Scheme

1: HBr / acetic acid / 0.17 h / Ambient temperature 2: N-ethylpiperidine / dimethylformamide / 12 h / Ambient temperature View Scheme

1: tetrahydrofuran / 1.) 3 h, 0 deg C; 2.) 12 h, r.t. 2: HBr / acetic acid / 0.17 h / Ambient temperature 3: N-ethylpiperidine / dimethylformamide / 12 h / Ambient temperature View Scheme

1: dicyclohexylcarbodiimide / tetrahydrofuran / 3 h / 0 °C 2: tetrahydrofuran / 1.) 3 h, 0 deg C; 2.) 12 h, r.t. 3: HBr / acetic acid / 0.17 h / Ambient temperature 4: N-ethylpiperidine / dimethylformamide / 12 h / Ambient temperature View Scheme

1-ethyl-piperidine in N,N-dimethyl-formamide 12 h; Ambient temperature; Yield given;