peg-NADH; formate dehydrogenase; D-hydroxyisocaproate dehydrogenase (D-HicDH) from Lactobacillus casei continuous production in an enzyme membrane reactor;

1: 100 percent / H2 / 5percent Pd/C / ethanol / 3750.3 Torr 2: 1.) K2CO3, EtOH, 2.) KOH / 1.) reflux, 5 h, 2.) 12 h 3: 96 percent / tetrahydrofuran / 48 h / 50 °C 5: 94 percent / DMAP, DCC / CH2Cl2 / 12 h / -20 - 20 °C 6: 92 percent / Pd(PPh3)4, morpholine / tetrahydrofuran / Ambient temperature View Scheme