NH4OH-NH4Cl buffer; pyridoxal 5'-phosphate T=30°C; 0.5 h; relative rate of CO2 evolution by aromatic L-amino acid decarboxylase from Micrococcus percitreus;
1: With manganese (II) chloride in phosphate buffer T=25°C; pH=5.5; 24 h; 2: With Pseudomonas putida L-threonine aldolase in phosphate buffer pH=5.5; 100 h; Further stages.;