Tris buffer; water; subtilisin var. of enzyme, pH, further in D2O, D2O-H2O; Kinetics;

(R)-α-lt;((2S,3S)-2-(9-fluorenylmethyloxycarbonylamino)-3-hydroxybutanoyl)aminogt;benzenethaneboronic acid; sodium chloride; α-chymotrypsin; 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid T=25°C; other boronic acid inhibitors; Rate constant;