1: tetrahydrofuran / 0 - 20 °C 2: 2 h / 20 °C 3: 65 percent / N-methylmorpholine; isobutyl chloroformate; sodium borohydride / tetrahydrofuran / 3 h / -20 °C 4: 95 percent / iodine; triphenylphosphane; imidazole / CH2Cl2 / 2 h 5: 73 percent / triethylamine; hydrogen / Pd/C / methanol / 3 h View Scheme

in water T=20°C; Michaelis and catalytic center activity constants for deamination reaction by 3-methylaspartase from Morganella morganii strain YG-0601; pH 9.7;