sodium acetate; zinc(II) chloride T=37°C;

1: 96 percent / TRIS buffer; conc. HCl; ZnCl2 / carboxypeptidase G / H2O / 48 h / 38 °C / pH 7.2 2: 83 percent / diphenylphosphoryl azide; Et3N / dimethylsulfoxide / 20 h 3: 100 percent / tetramethylguanidine / dimethylsulfoxide / 1 h / 25 °C View Scheme