1: AcOH / H2O / 1.) steam bath, 6 h, 2.) room temperature, overnight 2: 19.12 g / AcOH / 4 h 3: 5.89 g / H2O, conc. aq. NH4OH, porcine renal acylase I, cobaltous acetate tetrahydrate / 96 h / 37 °C / pH 7-7.5 View Scheme

ammonium hydroxide; porcine renal acylase I; water; anhydrous cobalt diacetate T=37°C; 96 h; pH 7-7.5; Yield given;

1: 19.12 g / AcOH / 4 h 2: 5.89 g / H2O, conc. aq. NH4OH, porcine renal acylase I, cobaltous acetate tetrahydrate / 96 h / 37 °C / pH 7-7.5 View Scheme

acetic acid 4 h; Yields of byproduct given;