sodium carbonate; penicillin G amidase in water T=29 - 33°C; pH=8; Large scale reactionEnzymatic reaction;

1: With triethylamine in tetrahydrofuran; water T=20°C; 4 - 6 h; 2: With potassium carbonate in water T=20°C; pH=8.0 - 8.2; 0.5 - 0.75 h; 3: With sulfuric acid in water pH=2 - 2.5;

N,O-bis-(trimethylsilyl)-acetamide in dichloromethane T=0 - 20°C; 3.5 h;