1: With methanol; ammonia; water; hydrolase SEQ ID NO:4 (encoded by SEQ ID NO:3) in toluene T=50°C; pH=6.75; tris bufferEnzymatic reaction; 2: With hydrogenchloride in water pH=2.5; Product distribution / selectivity;
1: With water; hydrogenchloride in dichloromethane T=0 - 5°C; 2: With water; sodium hydrogencarbonate in toluene; acetonitrile Product distribution / selectivity;
methanol; ammonia; water; esterase SEQ ID NO:4 (encoded by SEQ ID NO:3) in toluene pH=7; Tris bufferEnzymatic reaction; Product distribution / selectivity;
1: With methylamine in water; acetonitrile T=5 - 20°C; 0.5 - 1 h; 2: With hydrogenchloride; water in acetonitrile T=0 - 5°C; Product distribution / selectivity;
