hydrogen; acetic acid; palladium on activated charcoal in methanol; N,N-dimethyl-formamide 2 h; Ambient temperature;

arylsulfate sulfotransferase from Desulfitobacterium hafniense in aq. buffer T=30°C; pH=8; 144 h; Green chemistryEnzymatic reaction; regioselective reaction;

1: N-Methylmorpholine 2: 79 percent / triethylamine / dimethylformamide 3: 100 percent / DMF*SO3 / dimethylformamide; pyridine / 2 h / 30 °C / other reagent (Pyr*SO3) 4: 66 percent / AcOH, H2 / Pd/C (5percent) / dimethylformamide; methanol / 2 h / Ambient temperature View Scheme

1: 79 percent / triethylamine / dimethylformamide 2: 100 percent / DMF*SO3 / dimethylformamide; pyridine / 2 h / 30 °C / other reagent (Pyr*SO3) 3: 66 percent / AcOH, H2 / Pd/C (5percent) / dimethylformamide; methanol / 2 h / Ambient temperature View Scheme

1: 79 percent / triethylamine / dimethylformamide 2: 100 percent / DMF*SO3 / dimethylformamide; pyridine / 2 h / 30 °C / other reagent (Pyr*SO3) 3: 66 percent / AcOH, H2 / Pd/C (5percent) / dimethylformamide; methanol / 2 h / Ambient temperature View Scheme

1: CF3COOH / various solvent(s) 2: 79 percent / triethylamine / dimethylformamide 3: 100 percent / DMF*SO3 / dimethylformamide; pyridine / 2 h / 30 °C / other reagent (Pyr*SO3) 4: 66 percent / AcOH, H2 / Pd/C (5percent) / dimethylformamide; methanol / 2 h / Ambient temperature View Scheme

1: 100 percent / DMF*SO3 / dimethylformamide; pyridine / 2 h / 30 °C / other reagent (Pyr*SO3) 2: 66 percent / AcOH, H2 / Pd/C (5percent) / dimethylformamide; methanol / 2 h / Ambient temperature View Scheme