1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 14: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 16: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 18: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 20: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 21: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 22: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 14: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 16: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 18: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

T=80°C; 0.333333 h; neat (no solvent);

1: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

18-crown-6 ether in acetonitrile T=20°C; Rate constantEquilibrium constant;

in n-heptane T=80°C; 48 h; Title compound not separated from byproducts;