silver trifluoroacetate; silica gel in ethyl acetate T=50°C; Wolff rearrangement; 0.25 h;

NIT-107 nitrilase; water in methanol T=30 - 32°C; aq. phosphate buffer; enantioselective reaction;

1.1: ClCOOEt; N-methylmorpholine / tetrahydrofuran / 0.25 h / -15 °C 1.2: tetrahydrofuran; CH2Cl2 / -15 - 20 °C 2.1: 70 percent / CF3COOAg / dioxane; H2O / 0.5 h / sonication View Scheme