phosphate buffer in ethanol T=37°C; baker's yeast (Saccharomyces cerevisiae, Sigma, Type 1);

in Petroleum ether T=18 - 25°C; 1.5 h;

1: 85 percent / triethylamine / diethyl ether / Ambient temperature 2: 86 percent / aq. phosphate buffer (pH 7.4) / ethanol / 37 °C / baker's yeast (Saccharomyces cerevisiae, Sigma, Type 1) View Scheme

hydrogen bromide T=100°C; 5 h; sealed tube;