Dithiothreitol; chalcone synthase, immobilized onto Ni-nitrilotriacetic acid agarose; malonyl-CoA synthetase, immobilized onto glutathione-sepharose; disodium malonate; ATP disodium salt; Coenzyme A; magnesium chloride T=22°C; pH=7.5; 72 h; Tris-HCl bufferEnzymatic reaction;

1: benzoic acid With diphenyl phosphoryl azide; triethylamine in N,N-dimethyl-formamide T=0°C; 2 h; 2: N-(2-mercaptoethyl)-acetamide in N,N-dimethyl-formamide 2 h;

暂无反应条件数据

暂无反应条件数据

Dithiothreitol; chalcone synthase, immobilized onto Ni-nitrilotriacetic acid agarose; malonyl-CoA synthetase, immobilized onto glutathione-sepharose; disodium malonate; ATP disodium salt; Coenzyme A; magnesium chloride T=22°C; pH=7.5; 72 h; Tris-HCl bufferEnzymatic reaction;