1: aq. NaOH, 30percent aq. H2O2 / 1 h / 40 °C 2: 1.) 3N aq. NaOH; 2.) 4N aq. H2SO4 / 1.) ca 100 deg C, 20 h; 2.) cool with ice View Scheme

halohydrin dehalogenase (Agrobacterium radiobacter AD1,HheC); Tris-SO4 buffer in dimethyl sulfoxide T=22°C; pH=7.5; 5 h;

Tris-SO4 buffer in water; dimethyl sulfoxide T=22°C; pH=7.5; 5 h; Title compound not separated from byproducts.;

borane in tetrahydrofuran 1.) add BH3 over 14 min, 0 grad C; 2.) stir 2 h; Yield given;

1: pyridine / 16 h / 0 °C 2: 60 percent / ethanol; H2O / 1.) add KCN, 0 grad C; 2.) stir overnight, room temp. 3: BH3 / tetrahydrofuran / 1.) add BH3 over 14 min, 0 grad C; 2.) stir 2 h View Scheme

1: LiAlH4 / diethyl ether; tetrahydrofuran / 24 h 2: pyridine / 16 h / 0 °C 3: 60 percent / ethanol; H2O / 1.) add KCN, 0 grad C; 2.) stir overnight, room temp. 4: BH3 / tetrahydrofuran / 1.) add BH3 over 14 min, 0 grad C; 2.) stir 2 h View Scheme

1: N-chlorosuccinimide, AgNO3, 2,6-lutidine / acetonitrile / 5 min water bath cooling, 45 min stirring 2: LiAlH4 / diethyl ether; tetrahydrofuran / 24 h 3: pyridine / 16 h / 0 °C 4: 60 percent / ethanol; H2O / 1.) add KCN, 0 grad C; 2.) stir overnight, room temp. 5: BH3 / tetrahydrofuran / 1.) add BH3 over 14 min, 0 grad C; 2.) stir 2 h View Scheme

1: 60 percent / ethanol; H2O / 1.) add KCN, 0 grad C; 2.) stir overnight, room temp. 2: BH3 / tetrahydrofuran / 1.) add BH3 over 14 min, 0 grad C; 2.) stir 2 h View Scheme

halohydrin dehalogenase from Mycobacterium sp. GP1 (HheB); Tris-SO4 buffer in dimethyl sulfoxide T=22°C; pH=7.5; 5 h; Product distribution; Further Variations:Reagents;